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of presumed genes encoding beta-lactamases by sequence based screening of metagenomes derived from Antarcticmicrobial mats

Gastón Azziz, Matías Giménez, Héctor Romero, Patricia M. Valdespino-Castillo, Luisa I. Falcón, Lucas A. M. Ruberto, Walter P. Mac Cormack, Silvia Batista

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1128-1

摘要：

• Beta-lactamase genes were found in all samples from distant places in Antarctica.

• Class C beta-lactamase coding genes were the most frequently found.

• Diversity of sequences exceeds that of the beta-lactamases from clinical environment.

关键词： Beta-lactamases     Antibiotic resistance coding genes     Metagenomes     Antarctic microbial mats    

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南极海洋保护区事务的发展及挑战

付玉

《中国工程科学》 2019年 第21卷 第6期   页码 9-17 doi: 10.15302/J-SSCAE-2019.06.002

摘要：

南极海洋保护区事务近 10年来发展较快，是南极海洋治理中备受关注的重点议题。本文介绍了南极海洋保护区的设立进程，探讨了南极海洋保护区事务面临的挑战，提出了中国参与南极海洋治理的建议。南极海洋生物资源养护委员会各成员方在南极海洋保护区法律制度、政治因素、科学基础和管理监测等方面仍存在分歧和矛盾，是南极海洋保护区事务进一步发展的主要挑战。为实施“海洋命运共同体”重要理念，履行南极海洋生物资源养护委员会成员国义务，中国应深度参与南极海洋保护区事务、尽快完善国内海洋保护区法律制度，并大力加强南极海洋生态环境科学研究。

关键词： 南极海洋保护区     南极海洋治理与养护     科学数据    

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南极磷虾渔业发展的工程科技需求

赵宪勇,左涛,冷凯良,唐启升

《中国工程科学》 2016年 第18卷 第2期   页码 85-90 doi: 10.15302/J-SSCAE-2016.02.010

摘要：

积极参与开发南极海洋生物资源是对海洋渔业持续健康发展的新要求。本文介绍了国际南极磷虾渔业的发展历程与趋势，分析了我国南极磷虾渔业面临的主要问题，并提出了相关工程科技建议。国际上南极磷虾渔业已发展成为由创新性捕捞技术支撑、高附加产品市场拉动、集捕捞和加工于一体的新型产业。积极发展南极磷虾渔业，可促进我国第二远洋渔业发展、培育海洋生物新兴产业。为实现这一目标，需加强捕捞加工装备技术创新研究，提高产业核心竞争力；同时开展渔场资源生态基础研究，促进极地渔业可持续发展。

关键词： 南极磷虾     工程科技     极地渔业     新兴产业    

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极地系统科学考察——大科学系统工程管理探索

陈立奇

《中国工程科学》 2004年 第6卷 第2期   页码 1-7

摘要：

人与地球系统之间的相互作用已成为全球变化的重要驱动力，极地系统是感应全球变化的放大器和指示器极地系统科学进入了一个大科学研究时代。极地系统科学不但是一门研究极地系统对全球变化的响应与反馈作用中运转的机制、规律和控制这些变化的机理，并为区域复杂性及其对全球变化预测提供科学基础的科学，同时也是一门高新技术应用和人类认识自然活动并科学管理极地系统的系统工程学。20年来，中国科学家对极地系统复杂性有了深入的认识，对极地科学考察管理也进行了有益的探索。

关键词： 全球变化科学     极地系统科学     系统工程管理    

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Responses of microbial interactions to elevated salinity in activated sludge microbial community

《环境科学与工程前沿（英文）》 2023年 第17卷 第5期 doi: 10.1007/s11783-023-1660-x

摘要：

● Salinity led to the elevation of NAR over 99.72%.

关键词： Elevated salinity     Activated sludge system     Pollution removal     Microbial interactions     Competitive relationship    

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我国南极磷虾捕捞与加工装备科技发展研究

谌志新,王志勇,欧阳杰

《中国工程科学》 2019年 第21卷 第6期   页码 48-52 doi: 10.15302/J-SSCAE-2019.06.008

摘要：

南极磷虾资源具有巨大的开发和利用潜力，积极参与南极磷虾渔业开发，可有效促进我国远洋渔业的发展。专业性的捕捞和加工装备技术成为现代南极磷虾渔业的基本特征，是南极磷虾高效开发利用的重要保障。本文详细介绍了国外先进磷虾船装备的发展和应用现状，以及我国在南极磷虾捕捞装备和虾粉、虾油设备研发方面取得的进展，分析了国内在南极磷虾开发专业装备方面存在的差距和原因。针对我国南极磷虾船装备存在专业化程度低、生产工艺落后、缺乏核心技术等问题，在磷虾生态高效捕捞、精深加工工艺和资源探测评估技术方面提出重点研究方向，为我国南极磷虾高效开发和综合利用提供对策和建议。

关键词： 南极磷虾     开发装备     生态捕捞     精深加工     资源探测    

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Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 21-27 doi: 10.1007/s11783-011-0303-9

摘要： New high-throughput technologies continue to emerge for studying complex microbial communities. In particular, massively parallel pyrosequencing enables very high numbers of sequences, providing a more complete view of community structures and a more accurate inference of the functions than has been possible just a few years ago. In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring of specific community members over time, space, or different environmental conditions. In this review, the principles of these two methods and their complementary applications in studying microbial ecology in bioenvironmental systems are discussed. The parallel sequencing of amplicon libraries and using barcodes to differentiate multiple samples in a pyrosequencing run are explained. The best procedures and chemistries for QPCR amplifications are also described and advantages of applying automation to increase accuracy are addressed. Three examples in which pyrosequencing and QPCR were used together to define and quantify members of microbial communities are provided: in the human large intestine, in a methanogenic digester whose sludge was made more bioavailable by a high-voltage pretreatment, and on the biofilm anode of a microbial electrolytic cell. The key findings in these systems and how both methods were used in concert to achieve those findings are highlighted.

关键词： polymerase chain reaction (PCR)     microbial communities     pyrosequencing     gut     microbial fuel cell     sludge    

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LINKING CROP WATER PRODUCTIVITY TO SOIL PHYSICAL, CHEMICAL AND MICROBIAL PROPERTIES

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 545-558 doi: 10.15302/J-FASE -2020349

摘要：

Agriculture uses a large proportion of global and regional water resources. Due to the rapid increase of population in the world, the increasing competition for water resources has led to an urgent need in increasing crop water productivity for agricultural sustainability. As the medium for crop growth, soils and their properties are important in affecting crop water productivity. This review examines the effects of soil physical, chemical, and microbial properties on crop water productivity and the quantitative relationships between them. A comprehensive view of these relationships may provide important insights for soil and water management in arable land for agriculture in the future.

 

关键词： crop water productivity     crop yield     soil chemical properties     soil microbial properties     soil physical properties     water consumption    

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thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbial

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1342-x

摘要：

• SMX addition had negative effect on acetoclastic methanogens in mesophilic AD.

关键词： Pig manure     Antibiotics     Anaerobic digestion     Resistance genes     Microbial community    

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porphyrinic triazine-based frameworks with excellent biocompatibility for conversion of CO in H-mediated microbial

《化学科学与工程前沿（英文）》 2022年 第16卷 第12期   页码 1761-1771 doi: 10.1007/s11705-022-2195-6

摘要： Microbial electrosynthesis is a promising alternative to directly convert CO2 into long-chain compounds by coupling inorganic electrocatalysis with biosynthetic systems. However, problems arose that the conventional electrocatalysts for hydrogen evolution may produce extensive by-products of reactive oxygen species and cause severe metal leaching, both of which induce strong toxicity toward microorganisms. Moreover, poor stability of electrocatalysts cannot be qualified for long-term operation. These problems may result in poor biocompatibility between electrocatalysts and microorganisms. To solve the bottleneck problem, Co anchored on porphyrinic triazine-based frameworks was synthesized as the electrocatalyst for hydrogen evolution and further coupled with Cupriavidus necator H16. It showed high selectivity for a four-electron pathway of oxygen reduction reaction and low production of reactive oxygen species, owing to the synergistic effect of Co–Nx modulating the charge distribution and adsorption energy of intermediates. Additionally, low metal leaching and excellent stability were observed, which may be attributed to low content of Co and the stabilizing effect of metalloporphyrins. Hence, the electrocatalyst exhibited excellent biocompatibility. Finally, the microbial electrosynthesis system equipped with the electrocatalyst successfully converted CO2 to poly-β-hydroxybutyrate. This work drew up a novel strategy for enhancing the biocompatibility of electrocatalysts in microbial electrosynthesis system.

关键词： microbial electrosynthesis     hydrogen evolution reaction     metalloporphyrins     biocompatibility     CO2 conversion    

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Microbial electrolysis cells with biocathodes and driven by microbial fuel cells for simultaneous enhanced

Jingya SHEN,Yuliang SUN,Liping HUANG,Jinhui YANG

《环境科学与工程前沿（英文）》 2015年 第9卷 第6期   页码 1084-1095 doi: 10.1007/s11783-015-0805-y

摘要： Cobalt and copper recovery from aqueous Co(II) and Cu(II) is one critical step for cobalt and copper wastewaters treatment. Previous tests have primarily examined Cu(II) and Co(II) removal in microbial electrolysis cells (MECs) with abiotic cathodes and driven by microbial fuel cell (MFCs). However, Cu(II) and Co(II) removal rates were still slow. Here we report MECs with biocathodes and driven by MFCs where enhanced removal rates of 6.0±0.2 mg?L ?h for Cu(II) at an initial concentration of 50 mg?L and 5.3±0.4 mg?L h for Co(II) at an initial 40 mg?L were achieved, 1.7 times and 3.3 times as high as those in MECs with abiotic cathodes and driven by MFCs. Species of Cu(II) was reduced to pure copper on the cathodes of MFCs whereas Co(II) was removed associated with microorganisms on the cathodes of the connected MECs. Higher Cu(II) concentrations and smaller working volumes in the cathode chambers of MFCs further improved removal rates of Cu(II) (115.7 mg?L ?h ) and Co(II) (6.4 mg?L ?h ) with concomitantly achieving hydrogen generation (0.05±0.00 mol?mol COD). Phylogenetic analysis on the biocathodes indicates dominantly accounted for 67.9% of the total reads, followed by (14.0%), (6.1%), (2.5%), (1.4%), and (1.0%). This study provides a beneficial attempt to achieve simultaneous enhanced Cu(II) and Co(II) removal, and efficient Cu(II) and Co(II) wastewaters treatment without any external energy consumption.

关键词： biocathode     microbial electrolysis cell     microbial fuel cell     Cu(II) removal     Co(II) removal    

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Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

《环境科学与工程前沿（英文）》 2012年 第6卷 第1期   页码 82-97 doi: 10.1007/s11783-011-0306-6

摘要： Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.

关键词： molecular biological technique     microbial community     denaturing gradient gel electrophoresis (DGGE)     terminal restriction fragment length polymorphism (T-RFLP)     environmental applications    

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Comparison of the removal of monovalent and divalent cations in the microbial desalination cell

Shanshan CHEN,Haiping LUO,Yanping HOU,Guangli LIU,Renduo ZHANG,Bangyu QIN

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 317-323 doi: 10.1007/s11783-013-0596-y

摘要： Microbial desalination cell (MDC) is a promising technology to desalinate water and generate electrical power simultaneously. The objectives of this study were to investigate the desalination performance of monovalent and divalent cations in the MDC, and discuss the effect of ion characteristics, ion concentrations, and electrical characteristics. Mixed salt solutions of NaCl, MgCl , KCl, and CaCl with the same concentration were used in the desalination chamber to study removal of cations. Results showed that in the mixed salt solutions, the electrodialysis desalination rates of cations were: Ca >Mg >Na >K . Higher ionic charges and smaller hydrated ionic radii resulted in higher desalination rates of the cations, in which the ionic charge was more important than the hydrated ionic radius. Mixed solutions of NaCl and MgCl with different concentrations were used in the desalination chamber to study the effect of ion concentrations. Results showed that when ion concentrations of Na were one-fifth to five times of Mg , ion concentration influenced the dialysis more profoundly than electrodialysis. With the current densities below a certain value, charge transfer efficiencies became very low and the dialysis was the main process responsible for the desalination. And the phosphate transfer from the anode chamber and potassium transfer from the cathode chamber could balance 1%–3% of the charge transfer in the MDC.

关键词： divalent ion     electrodialysis     ion characteristic     microbial desalination cell     monovalent ion    

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Water-dispersible nano-pollutions reshape microbial metabolism in type-specific manners: A metabolic

《环境科学与工程前沿（英文）》 2022年 第16卷 第9期 doi: 10.1007/s11783-022-1548-1

摘要：

• Water-dispersible nano-pollutions exhibit type-specific toxic effects on E. coli.

关键词： Nano-toxicity     Nano-plastics     Quantum dots     Microbial metabolite     Metabolic dysregulation    

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Repeated batch fermentation with water recycling and cell separation for microbial lipid production

Yumei WANG, Wei LIU, Jie BAO

《化学科学与工程前沿（英文）》 2012年 第6卷 第4期   页码 453-460 doi: 10.1007/s11705-012-1210-8

摘要： Large waste water disposal was the major problem in microbial lipid fermentation because of low yield of lipid. In this study, the repeated batch fermentation was investigated for reducing waste water generated in the lipid fermentation of an oleaginous yeast CX1 strain. The waste fermentation broth was recycled in the next batch operation after the cells were separated using two different methods, centrifugation and flocculation. Two different sugar substrates, glucose and inulin, were applied to the proposed operation. The result showed that at least 70% of the waste water was reduced, while lipid production maintained satisfactory in the initial four cycles. Furthermore, it is suggested that CX1 cells might produce certain naturally occurring inulin hydrolyzing enzyme(s) for obtaining fructose and glucose from inulin directly. Our method provided a practical option for reducing the waste water generated from microbial lipid fermentation.

关键词： batch fermentation     microbial lipid     Trichosporon cutaneum CX1     flocculation     waste water recycle    

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标题 作者 时间 类型 操作

of presumed genes encoding beta-lactamases by sequence based screening of metagenomes derived from Antarcticmicrobial mats

Gastón Azziz, Matías Giménez, Héctor Romero, Patricia M. Valdespino-Castillo, Luisa I. Falcón, Lucas A. M. Ruberto, Walter P. Mac Cormack, Silvia Batista

期刊论文

南极海洋保护区事务的发展及挑战

付玉

期刊论文

南极磷虾渔业发展的工程科技需求

赵宪勇,左涛,冷凯良,唐启升

期刊论文

极地系统科学考察——大科学系统工程管理探索

陈立奇

期刊论文

Responses of microbial interactions to elevated salinity in activated sludge microbial community

期刊论文

我国南极磷虾捕捞与加工装备科技发展研究

谌志新,王志勇,欧阳杰

期刊论文

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

期刊论文

LINKING CROP WATER PRODUCTIVITY TO SOIL PHYSICAL, CHEMICAL AND MICROBIAL PROPERTIES

期刊论文

thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbial

期刊论文

porphyrinic triazine-based frameworks with excellent biocompatibility for conversion of CO in H-mediated microbial

期刊论文

Microbial electrolysis cells with biocathodes and driven by microbial fuel cells for simultaneous enhanced

Jingya SHEN,Yuliang SUN,Liping HUANG,Jinhui YANG

期刊论文

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

期刊论文

Comparison of the removal of monovalent and divalent cations in the microbial desalination cell

Shanshan CHEN,Haiping LUO,Yanping HOU,Guangli LIU,Renduo ZHANG,Bangyu QIN

期刊论文

Water-dispersible nano-pollutions reshape microbial metabolism in type-specific manners: A metabolic

期刊论文

Repeated batch fermentation with water recycling and cell separation for microbial lipid production

Yumei WANG, Wei LIU, Jie BAO

期刊论文